5ZBU

Crystal Structure of PA-TM-RING E3 ligase RNF13 RING domain in complex with E2~Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, B	Ubiquitin-conjugating enzyme E2 D2	polymer	148	16838.3	2	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	A, D	E3 ubiquitin-protein ligase RNF13	polymer	76	8840.5	2	UniProt (O43567) Pfam (PF13639) In PDB	Homo sapiens (Human)	RING finger protein 13,RING-type E3 ubiquitin transferase RNF13
3	E	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
4	A, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5		water	water		18.0	9	Chemie (HOH)

Sequence modifications

C, B: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly 0	-	expression tag
Lys 85	Cys 85	engineered mutation

A, D: 216 - 290 (UniProt: O43567)

PDB	External Database	Details
Met 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	59934.4
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	60196.1

*Water molecules are not included.