5Z6H

Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Periplasmic trehalase	polymer	563	63167.5	2	UniProt (A0A156C5X3)	Enterobacter cloacae	Alpha,alpha-trehalase,alpha-trehalose glucohydrolase
2	C, D, E, F, G... (A, B)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
3	I, N (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	O, P (A, B)	water	water		18.0	777	Chemie (HOH)

Sequence modifications

A, B: 30 - 556 (UniProt: A0A156C5X3)

PDB	External Database	Details
Met -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
Gly 6	-	expression tag
Leu 7	-	expression tag
Val 8	-	expression tag
Pro 9	-	expression tag
Arg 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
Met 14	-	expression tag
Ala 15	-	expression tag
Ser 16	-	expression tag
Met 17	-	expression tag
Thr 18	-	expression tag
Gly 19	-	expression tag
Gly 20	-	expression tag
Gln 21	-	expression tag
Gln 22	-	expression tag
Met 23	-	expression tag
Gly 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
Glu 28	-	expression tag
Phe 29	-	expression tag
Asn 32	Ser 32	engineered mutation
Thr 35	Ala 35	engineered mutation
Arg 429	His 429	see sequence details
Val 553	Ala 553	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	126335.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1144.8
All*	Total formula weight	127479.9

*Water molecules are not included.