5Z6H

Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97937
Spacegroup name	P 21 21 21
Unit cell lengths	55.250, 127.909, 156.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.319 - 2.300
R-factor	0.1715
Rwork	0.168
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jg0
RMSD bond length	0.007
RMSD bond angle	0.926
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.320	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.121	0.592
Rmeas	0.139	0.678
Rpim	0.086	0.421
Number of reflections	48818	4363
<I/σ(I)>	9.5	2.5
Completeness [%]	97.9	96.8
Redundancy	4.2	4.2
CC(1/2)	0.980	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.5M Ammonium sulphate, 25% PEG 4000, 15% glycerol