5Z3G
Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 25S rRNA | polymer | 3396 | 1097493.9 | 1 | GenBank (834774822) | Saccharomyces cerevisiae (Baker's yeast) | |
| 2 | B (B) | 5.8S rRNA | polymer | 158 | 50682.9 | 1 | GenBank (1267176494) | Saccharomyces cerevisiae (Baker's yeast) | |
| 3 | C (C) | ITS2 RNA | polymer | 232 | 74308.4 | 1 | GenBank (1104417410) | Saccharomyces cerevisiae (Baker's yeast) | |
| 4 | D (D) | Ribosome biogenesis protein RLP7 | polymer | 322 | 36621.1 | 1 | UniProt (P40693) Pfam (PF00327) | Saccharomyces cerevisiae S288c (Baker's yeast) | Ribosomal protein L7-like |
| 5 | E (E) | Ribosome biogenesis protein 15 | polymer | 220 | 25499.2 | 1 | UniProt (P53927) Pfam (PF00076) | Saccharomyces cerevisiae S288c (Baker's yeast) | Nucleolar protein 15 |
| 6 | F (F) | 60S ribosomal protein L3 | polymer | 387 | 43850.8 | 1 | UniProt (P14126) Pfam (PF00297) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein uL3,Maintenance of killer protein 8,RP1,Trichodermin resistance protein,YL1 |
| 7 | G (G) | 60S ribosomal protein L4-A | polymer | 362 | 39159.1 | 1 | UniProt (P10664) Pfam (PF00573) Pfam (PF14374) | Saccharomyces cerevisiae S288c (Baker's yeast) | L2,Large ribosomal subunit protein uL4-A,RP2,YL2 |
| 8 | H (H) | Proteasome-interacting protein CIC1 | polymer | 376 | 42596.7 | 1 | UniProt (P38779) Pfam (PF00687) | Saccharomyces cerevisiae S288c (Baker's yeast) | Core interacting component 1 |
| 9 | I (I) | 60S ribosomal protein L6-A | polymer | 176 | 20000.6 | 1 | UniProt (Q02326) Pfam (PF01159) | Saccharomyces cerevisiae S288c (Baker's yeast) | L17,Large ribosomal subunit protein eL6-A,RP18,YL16 |
| 10 | J (J) | 60S ribosomal protein L7-A | polymer | 244 | 27686.3 | 1 | UniProt (P05737) Pfam (PF08079) Pfam (PF00327) | Saccharomyces cerevisiae S288c (Baker's yeast) | L6,Large ribosomal subunit protein uL30-A,RP11,YL8 |
| 11 | K (K) | 60S ribosomal protein L8-A | polymer | 256 | 28175.8 | 1 | UniProt (P17076) Pfam (PF01248) | Saccharomyces cerevisiae S288c (Baker's yeast) | L4,L4-2,L7a-1,Large ribosomal subunit protein eL8-A,Maintenance of killer protein 7,RP6,YL5 |
| 12 | L (L) | 60S ribosomal protein L9-A | polymer | 191 | 21605.1 | 1 | UniProt (P05738) Pfam (PF00347) | Saccharomyces cerevisiae S288c (Baker's yeast) | L8,Large ribosomal subunit protein uL6-A,RP24,YL11 |
| 13 | M (M) | Nucleolar protein 7 | polymer | 605 | 69984.1 | 1 | UniProt (P53261) Pfam (PF06732) Pfam (PF16589) | Saccharomyces cerevisiae S288c (Baker's yeast) | Pescadillo homolog,Ribosomal RNA-processing protein 13 |
| 14 | N (N) | Eukaryotic translation initiation factor 6 | polymer | 245 | 26476.6 | 1 | UniProt (Q12522) Pfam (PF01912) | Saccharomyces cerevisiae S288c (Baker's yeast) | eIF-6 |
| 15 | O (O) | Ribosome production factor 1 | polymer | 295 | 35184.4 | 1 | UniProt (P38805) Pfam (PF04427) | Saccharomyces cerevisiae S288c (Baker's yeast) | Ribosome biogenesis protein RPF1 |
| 16 | P (P) | 60S ribosomal protein L13-A | polymer | 199 | 22604.2 | 1 | UniProt (Q12690) Pfam (PF01294) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein eL13-A |
| 17 | Q (Q) | 60S ribosomal protein L14-A | polymer | 138 | 15195.1 | 1 | UniProt (P36105) Pfam (PF00467) Pfam (PF01929) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein eL14-A |
| 18 | R (R) | 60S ribosomal protein L15-A | polymer | 204 | 24482.4 | 1 | UniProt (P05748) Pfam (PF00827) | Saccharomyces cerevisiae S288c (Baker's yeast) | L13,Large ribosomal subunit protein eL15-A,RP15R,YL10,YP18 |
| 19 | S (S) | 60S ribosomal protein L16-A | polymer | 199 | 22247.2 | 1 | UniProt (P26784) Pfam (PF00572) | Saccharomyces cerevisiae S288c (Baker's yeast) | L13a,L21,Large ribosomal subunit protein uL13-A,RP22,YL15 |
| 20 | T (T) | 60S ribosomal protein L17-A | polymer | 184 | 20589.5 | 1 | UniProt (P05740) Pfam (PF00237) | Saccharomyces cerevisiae S288c (Baker's yeast) | L20A,Large ribosomal subunit protein uL22-A,YL17 |
| 21 | U (U) | 60S ribosomal protein L18-A | polymer | 186 | 20609.3 | 1 | UniProt (P0CX49) Pfam (PF17135) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein eL18-A,RP28 |
| 22 | V (V) | Ribosome biogenesis protein NSA1 | polymer | 463 | 51982.5 | 1 | UniProt (P53136) | Saccharomyces cerevisiae S288c (Baker's yeast) | NOP7-associated protein 1 |
| 23 | W (W) | 60S ribosomal protein L20-A | polymer | 172 | 20478.9 | 1 | UniProt (P0CX23) Pfam (PF01775) | Saccharomyces cerevisiae S288c (Baker's yeast) | L18a,Large ribosomal subunit protein eL20-A |
| 24 | X (X) | Ribosomal RNA-processing protein 1 | polymer | 278 | 33250.5 | 1 | UniProt (P35178) Pfam (PF05997) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 25 | Y (Y) | ATP-dependent RNA helicase HAS1 | polymer | 505 | 56798.3 | 1 | UniProt (Q03532) Pfam (PF00270) Pfam (PF00271) Pfam (PF13959) | Saccharomyces cerevisiae S288c (Baker's yeast) | Helicase associated with SET1 protein 1 |
| 26 | Z (Z) | Protein MAK16 | polymer | 306 | 35754.6 | 1 | UniProt (P10962) Pfam (PF01778) Pfam (PF04874) | Saccharomyces cerevisiae S288c (Baker's yeast) | Maintenance of killer protein 16 |
| 27 | AA (a) | Ribosome biogenesis protein BRX1 | polymer | 291 | 33585.4 | 1 | UniProt (Q08235) Pfam (PF04427) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 28 | BA (b) | rRNA-processing protein EBP2 | polymer | 427 | 49842.6 | 1 | UniProt (P36049) Pfam (PF05890) | Saccharomyces cerevisiae S288c (Baker's yeast) | EBNA1-binding protein homolog |
| 29 | CA (c) | 60S ribosomal protein L26-A | polymer | 127 | 14265.8 | 1 | UniProt (P05743) Pfam (PF16906) Pfam (PF00467) | Saccharomyces cerevisiae S288c (Baker's yeast) | L33,Large ribosomal subunit protein uL24-A,YL33 |
| 30 | DA (d) | Unassigned | polymer | 154 | 13124.2 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
| 31 | EA (i) | 60S ribosomal protein L32 | polymer | 130 | 14809.4 | 1 | UniProt (P38061) Pfam (PF01655) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein eL32 |
| 32 | FA (j) | 60S ribosomal protein L33-A | polymer | 107 | 12177.1 | 1 | UniProt (P05744) Pfam (PF01247) | Saccharomyces cerevisiae S288c (Baker's yeast) | L37,Large ribosomal subunit protein eL33-A,RP47,YL37 |
| 33 | GA (l) | 60S ribosomal protein L35-A | polymer | 120 | 13942.6 | 1 | UniProt (P0CX84) Pfam (PF00831) | Saccharomyces cerevisiae S288c (Baker's yeast) | Large ribosomal subunit protein uL29-A |
| 34 | HA (m) | 60S ribosomal protein L36-A | polymer | 100 | 11151.3 | 1 | UniProt (P05745) Pfam (PF01158) | Saccharomyces cerevisiae S288c (Baker's yeast) | L39,Large ribosomal subunit protein eL36-A,YL39 |
| 35 | IA (n) | 60S ribosomal protein L37-A | polymer | 88 | 9877.4 | 1 | UniProt (P49166) Pfam (PF01907) | Saccharomyces cerevisiae S288c (Baker's yeast) | L43,Large ribosomal subunit protein eL37-A,YL35,YP55 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 2136093.1 | |
| All* | Total formula weight | 2136093.1 |
