5Z2U
ThDP-Mn2+ complex of R395A variant of EcMenD soaked with 2-ketoglutarate for 5 min
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... | 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase | polymer | 556 | 61347.7 | 8 | UniProt (P17109) Pfam (PF02776) Pfam (PF16582) Pfam (PF02775) In PDB | Escherichia coli (strain K12) | SEPHCHC synthase,Menaquinone biosynthesis protein MenD |
| 2 | G, A, B, H, C... | (4S)-4-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3lambda~5~-thiazol-2-yl}-4-hydroxybutanoic acid | non-polymer | 527.4 | 7 | Chemie (TD6) | |||
| 3 | F, G, A, B, H... | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 4 | E, F, G, B, H... | FORMIC ACID | non-polymer | 46.0 | 10 | Chemie (FMT) | |||
| 5 | G, H, C, D | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | F | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 1 | Chemie (TPP) | |||
| 7 | F | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 8 | water | water | 18.0 | 3282 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 556 (UniProt: P17109)
| PDB | External Database | Details |
|---|---|---|
| Ala 395 | Arg 395 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 490781.3 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 5294.4 | |
| All* | Total formula weight | 496075.7 |
