5Z23

Crystal structure of the nucleosome containing a chimeric histone H3/CENP-A CATD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1,Histone H3-like centromeric protein A,Histone H3.1	polymer	141	16063.9	2	UniProt (P68431) UniProt (P49450) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,Centromere autoantigen A,Centromere protein A,CENP-A
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	167	18406.7	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	129	14358.2	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I, J (I, J)	DNA (146-MER)	polymer	146	45053.9	2		Homo sapiens

Sequence modifications

A, E: 0 - 74 (UniProt: P68431)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

A, E: 75 - 114 (UniProt: P49450)
A, E: 115 - 137 (UniProt: P68431)
B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Mse 51	Leu 52	engineered mutation
Mse 58	Leu 59	engineered mutation
Mse 93	Leu 94	engineered mutation
Gln 130	-	expression tag
Leu 131	-	expression tag
Ala 132	-	expression tag
Ile 133	-	expression tag
Arg 134	-	expression tag
Asn 135	-	expression tag
Asp 136	-	expression tag
Glu 137	-	expression tag
Glu 138	-	expression tag
Mse 139	-	expression tag
Asn 140	-	expression tag
Lys 141	-	expression tag
Leu 142	-	expression tag
Leu 143	-	expression tag
Gly 144	-	expression tag
Arg 145	-	expression tag
Val 146	-	expression tag
Thr 147	-	expression tag
Ile 148	-	expression tag
Ala 149	-	expression tag
Gln 150	-	expression tag
Gly 151	-	expression tag
Gly 152	-	expression tag
Val 153	-	expression tag
Leu 154	-	expression tag
Pro 155	-	expression tag
Asn 156	-	expression tag
Ile 157	-	expression tag
Gln 158	-	expression tag
Ala 159	-	expression tag
Val 160	-	expression tag
Leu 161	-	expression tag
Leu 162	-	expression tag
Pro 163	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	211118.8
All*	Total formula weight	211118.8

*Water molecules are not included.