5Z23
Crystal structure of the nucleosome containing a chimeric histone H3/CENP-A CATD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.641, 100.745, 173.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.884 - 2.730 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.810 |
| High resolution limit [Å] | 2.710 | 5.840 | 2.710 |
| Rmerge | 0.071 | 0.048 | 0.492 |
| Rmeas | 0.077 | 0.053 | 0.546 |
| Rpim | 0.030 | 0.020 | 0.233 |
| Total number of observations | 258570 | ||
| Number of reflections | 45350 | 4916 | 4180 |
| <I/σ(I)> | 15 | ||
| Completeness [%] | 96.5 | 99 | 90.3 |
| Redundancy | 5.7 | 6.7 | 4.8 |
| CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
