5Z23
Crystal structure of the nucleosome containing a chimeric histone H3/CENP-A CATD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.641, 100.745, 173.431 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.884 - 2.730 |
R-factor | 0.2055 |
Rwork | 0.203 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.010 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.810 |
High resolution limit [Å] | 2.710 | 5.840 | 2.710 |
Rmerge | 0.071 | 0.048 | 0.492 |
Rmeas | 0.077 | 0.053 | 0.546 |
Rpim | 0.030 | 0.020 | 0.233 |
Total number of observations | 258570 | ||
Number of reflections | 45350 | 4916 | 4180 |
<I/σ(I)> | 15 | ||
Completeness [%] | 96.5 | 99 | 90.3 |
Redundancy | 5.7 | 6.7 | 4.8 |
CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |