5YW0

Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with succinate and Fe(III)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Uncharacterized protein KdoO	polymer	318	36239.3	1	UniProt (B3DUR4) Pfam (PF11004) In PDB	Methylacidiphilum infernorum (isolate V4)	3-Deoxy-D-manno-oct-2-ulosonic acid hydroxylase,Kdo hydroxylase
2	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	5	Chemie (PG4)
3	A	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
6	A	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
7		water	water		18.0	221	Chemie (HOH)

Sequence modifications

A: 1 - 305 (UniProt: B3DUR4)

PDB	External Database	Details
Lys 306	-	expression tag
Leu 307	-	expression tag
Ala 308	-	expression tag
Ala 309	-	expression tag
Ala 310	-	expression tag
Leu 311	-	expression tag
Glu 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36239.3
Non-Polymers*	Number of molecules	12
	Total formula weight	1473.3
All*	Total formula weight	37712.6

*Water molecules are not included.