5YT3

Structure of the Human Mitogen-Activated Protein Kinase Kinase 1 S218D and S222D mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Mitogen-activated protein kinase kinase 1, isoform CRA_d	polymer	350	39293.2	4	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (Human)	cDNA FLJ58770,highly similar to Dual specificity mitogen-activated protein kinase kinase 1 (EC 2.7.12.2)
2	E, G, H, J (A, B, C, D)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
3	F, I (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	K, L, M, N (A, B, C, D)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A, B, C, D: 39 - 382 (UniProt: Q02750)

PDB	External Database	Details
Asp 218	Ser 218	engineered mutation
Asp 222	Ser 222	engineered mutation
Ala 292	Thr 292	engineered mutation
Ala 298	Ser 298	engineered mutation
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	157172.6
Non-Polymers*	Number of molecules	6
	Total formula weight	2073.4
All*	Total formula weight	159246.0

*Water molecules are not included.