5YT3

Structure of the Human Mitogen-Activated Protein Kinase Kinase 1 S218D and S222D mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0004672	molecular_function	protein kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation
C	0004672	molecular_function	protein kinase activity
C	0005524	molecular_function	ATP binding
C	0006468	biological_process	protein phosphorylation
D	0004672	molecular_function	protein kinase activity
D	0005524	molecular_function	ATP binding
D	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue ANP A 401

Chain	Residue
A	ALA76
A	GLN153
A	ASP190
A	LYS192
A	SER194
A	ASN195
A	LEU197
A	ASP208
A	MG402
A	GLY80
A	VAL82
A	ALA95
A	LYS97
A	MET143
A	GLU144
A	MET146
A	SER150

---

site_id	AC2
Number of Residues	3
Details	binding site for residue MG A 402

Chain	Residue
A	ASN195
A	ASP208
A	ANP401

---

site_id	AC3
Number of Residues	18
Details	binding site for residue ANP B 401

Chain	Residue
B	LEU74
B	GLY75
B	ALA76
B	GLY77
B	ASN78
B	GLY79
B	VAL82
B	ALA95
B	LYS97
B	GLU144
B	MET146
B	SER150
B	GLN153
B	LYS192
B	SER194
B	ASN195
B	LEU197
B	ASP208

---

site_id	AC4
Number of Residues	17
Details	binding site for residue ANP C 401

Chain	Residue
C	LEU74
C	GLY77
C	GLY80
C	VAL82
C	ALA95
C	LYS97
C	GLU144
C	MET146
C	SER150
C	GLN153
C	ASP190
C	LYS192
C	SER194
C	ASN195
C	LEU197
C	ASP208
C	MG402

---

site_id	AC5
Number of Residues	4
Details	binding site for residue MG C 402

Chain	Residue
C	SER194
C	ASN195
C	ASP208
C	ANP401

---

site_id	AC6
Number of Residues	18
Details	binding site for residue ANP D 401

Chain	Residue
D	LEU74
D	GLY75
D	GLY77
D	ASN78
D	VAL82
D	ALA95
D	LYS97
D	MET143
D	GLU144
D	MET146
D	SER150
D	GLN153
D	LYS192
D	SER194
D	ASN195
D	LEU197
D	CYS207
D	HOH502

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK

Chain	Residue	Details
A	LEU74-LYS97

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV

Chain	Residue	Details
A	ILE186-VAL198

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15543157","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17880056","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18951019","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19019675","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19706763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21310613","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	13
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"10409742","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---