5YQT

Crystal Structure of the L74F/M78V/I80V/L114F mutant of LEH complexed with cyclopentene oxide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (C, D, A, B, E...)	Limonene-1,2-epoxide hydrolase	polymer	155	17369.4	8	UniProt (Q9ZAG3) Pfam (PF07858)	Rhodococcus erythropolis (Arthrobacter picolinophilus)
2	I, J, K, L, M (C, D, A, B, H)	(1R,5S)-6-oxabicyclo[3.1.0]hexane	non-polymer		84.1	5	Chemie (3ZS)
3	N, O, P, Q, R... (C, D, A, B, E...)	water	water		18.0	410	Chemie (HOH)

Sequence modifications

C, D, A, B, E, F, G, H: 5 - 149 (UniProt: Q9ZAG3)

PDB	External Database	Details
Met -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Thr 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Phe 74	Leu 74	engineered mutation
Val 78	Met 78	engineered mutation
Val 80	Ile 80	engineered mutation
Phe 114	Leu 114	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	138955.0
Non-Polymers*	Number of molecules	5
	Total formula weight	420.6
All*	Total formula weight	139375.6

*Water molecules are not included.