5YQ2

Crystal structure of E.coli aminopeptidase N in complex with Puromycin aminonucleoside

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Aminopeptidase N	polymer	891	101331.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432)	Escherichia coli (strain K12)	Alpha-aminoacylpeptide hydrolase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C (A)	(2R,3R,4S,5S)-4-AMINO-2-[6-(DIMETHYLAMINO)-9H-PURIN-9-YL]-5-(HYDROXYMETHYL)TETRAHYDRO-3-FURANOL	non-polymer		294.3	1	Chemie (GMC) PubChem (16219893) PubChem (1599) PubChem (6020) PubChem (23615495) PubChem (25791572) PubChem (40613821) PubChem (71207615) PubChem (51340257) PubChem (51340725) PubChem (1265900) PubChem (57614974) PubChem (58828383) PubChem (71064997) PubChem (7059447) PubChem (7059449) PubChem (7059451) PubChem (7059453) PubChem (11231691) PubChem (15886958) PubChem (162904386)
4	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
5	F, G (A)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI) PubChem (9084)
6	H (A)	water	water		18.0	806	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101331.2
Non-Polymers*	Number of molecules	6
	Total formula weight	748.0
All*	Total formula weight	102079.2

*Water molecules are not included.