5YQ2

Crystal structure of E.coli aminopeptidase N in complex with Puromycin aminonucleoside

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Aminopeptidase N	polymer	891	101331.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432) In PDB	Escherichia coli (strain K12)	Alpha-aminoacylpeptide hydrolase
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	(2R,3R,4S,5S)-4-AMINO-2-[6-(DIMETHYLAMINO)-9H-PURIN-9-YL]-5-(HYDROXYMETHYL)TETRAHYDRO-3-FURANOL	non-polymer		294.3	1	Chemie (GMC)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
6		water	water		18.0	806	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101331.2
Non-Polymers*	Number of molecules	6
	Total formula weight	748.0
All*	Total formula weight	102079.2

*Water molecules are not included.