5YPD

Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105N mutant) in complex with Methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Methionine aminopeptidase	polymer	319	34453.7	1	UniProt (A5U6L5) Pfam (PF00557) In PDB	Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)	MetAP,Peptidase M
2	A	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	A	METHIONINE	non-polymer		149.2	1	Chemie (MET)
6	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7		water	water		18.0	160	Chemie (HOH)

Sequence modifications

A: 1 - 285 (UniProt: A5U6L5)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 105	Cys 105	engineered mutation
Ala 284	Cys 284	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34453.7
Non-Polymers*	Number of molecules	7
	Total formula weight	509.7
All*	Total formula weight	34963.4

*Water molecules are not included.