5YPD

Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105N mutant) in complex with Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-09-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	49.226, 44.909, 57.295
Unit cell angles	90.00, 94.17, 90.00

Refinement procedure

Resolution	28.570 - 1.620
R-factor	0.1461
Rwork	0.144
R-free	0.18180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yj3
RMSD bond length	0.021
RMSD bond angle	2.106
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.570	30.000	1.680
High resolution limit [Å]	1.620	3.490	1.620
Rmerge	0.044	0.037	0.200
Rmeas	0.062	0.052	0.283
Rpim	0.044	0.037	0.200
Number of reflections	29506	5852	5139
<I/σ(I)>	34
Completeness [%]	90.7	94.7	83.2
Redundancy	1.9	1.9	1.9
CC(1/2)	0.996	0.993	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 27% Peg 3350, 3% Glycerol