5YKI

Crystal structure of the engineered nine-repeat PUF domain in complex with cognate 9nt-RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pumilio homolog 1	polymer	415	48062.0	1	UniProt (Q14671) Pfam (PF00806) In PDB	Homo sapiens (Human)	Pumilio-1
2	C	RNA (5'-R(*UP*GP*UP*UP*GP*UP*AP*UP*A)-3')	polymer	9	2834.7	1		synthetic construct
3		water	water		18.0	144	Chemie (HOH)

Sequence modifications

A: 1 - 128 (UniProt: Q14671)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Gln -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

A: 129 - 243 (UniProt: Q14671)
A: 244 - 392 (UniProt: Q14671)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50896.7
All*	Total formula weight	50896.7

*Water molecules are not included.