5YKI
Crystal structure of the engineered nine-repeat PUF domain in complex with cognate 9nt-RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.91939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.045, 70.318, 97.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 2.250 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m8z |
RMSD bond length | 0.005 |
RMSD bond angle | 0.647 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.120 | 0.501 |
Number of reflections | 22954 | 1095 |
<I/σ(I)> | 17.6 | 2.7 |
Completeness [%] | 99.6 | 96.7 |
Redundancy | 9.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1M BIS-TRIS Propane, pH 8.8, 20% PEG MME 550 |