5YKI

Crystal structure of the engineered nine-repeat PUF domain in complex with cognate 9nt-RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91939
Spacegroup name	P 21 21 21
Unit cell lengths	68.045, 70.318, 97.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.250
R-factor	0.1988
Rwork	0.197
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m8z
RMSD bond length	0.005
RMSD bond angle	0.647
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.120	0.501
Number of reflections	22954	1095
<I/σ(I)>	17.6	2.7
Completeness [%]	99.6	96.7
Redundancy	9.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	0.1M BIS-TRIS Propane, pH 8.8, 20% PEG MME 550