5YEW

Structural basis for GTP hydrolysis and conformational change of mitofusin 1 in mediating mitochondrial fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitofusin-1,Mitofusin-1 fusion protein	polymer	421	47405.9	1	UniProt (Q8IWA4) Pfam (PF00350)	Homo sapiens (Human)	Fzo homolog,Transmembrane GTPase MFN1,Fzo homolog,Transmembrane GTPase MFN1
2	B, C (B, C)	Mitofusin-1,Mitofusin-1 fusion protein	polymer	421	47392.9	2	UniProt (Q8IWA4) Pfam (PF00350)	Homo sapiens (Human)	Fzo homolog,Transmembrane GTPase MFN1,Fzo homolog,Transmembrane GTPase MFN1
3	D, H, L (A, B, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	3	Chemie (GDP)
4	E, I, M (A, B, C)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	3	Chemie (BEF)
5	F, J, N (A, B, C)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
6	G, K, O (A, B, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

A: 1 - 364 (UniProt: Q8IWA4)

PDB	External Database	Details
Val 138	Thr 138	engineered mutation
Asn 139	Val 139	engineered mutation

A: 694 - 741 (UniProt: Q8IWA4)

PDB	External Database	Details
Gly 685	-	linker
Ser 686	-	linker
Gly 687	-	linker
Ser 688	-	linker
Gly 689	-	linker
Ser 690	-	linker
Gly 691	-	linker
Gly 692	-	linker
Ser 693	-	linker

B, C: 1 - 684 (UniProt: Q8IWA4)
B, C: 694 - 741 (UniProt: Q8IWA4)

PDB	External Database	Details
Gly 685	-	linker
Ser 686	-	linker
Gly 687	-	linker
Ser 688	-	linker
Gly 689	-	linker
Ser 690	-	linker
Gly 691	-	linker
Gly 692	-	linker
Ser 693	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	142191.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1717.8
All*	Total formula weight	143909.5

*Water molecules are not included.