5YEC

Crystal structure of Atg7CTD-Atg8-MgATP complex in form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-like modifier-activating enzyme ATG7	polymer	340	38150.9	2	UniProt (P38862) Pfam (PF00899)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ATG12-activating enzyme E1 ATG7,Autophagy-related protein 7,Cytoplasm to vacuole targeting protein 2
2	B, D (B, D)	Autophagy-related protein 8	polymer	119	13751.9	2	UniProt (P38182) Pfam (PF02991)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Autophagy-related ubiquitin-like modifier ATG8,Cytoplasm to vacuole targeting protein 5
3	E, H (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, I (A, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	G, J (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	K, L, M, N (A, B, C, D)	water	water		18.0	175	Chemie (HOH)

Sequence modifications

A, C: 295 - 630 (UniProt: P38862)

PDB	External Database	Details
Gly 291	-	expression tag
Pro 292	-	expression tag
His 293	-	expression tag
Met 294	-	expression tag

B, D: 1 - 116 (UniProt: P38182)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Pro 26	Lys 26	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	103805.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1193.8
All*	Total formula weight	104999.2

*Water molecules are not included.