5YC8

Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with NMS (Hg-derivative)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Muscarinic acetylcholine receptor M2,Redesigned apo-cytochrome b562,Muscarinic acetylcholine receptor M2	polymer	421	47293.2	1	UniProt (P08172) Pfam (PF00001) Pfam (PF07361) UniProt (by SIFTS) (P0ABE7) In PDB	Homo sapiens (Human)
2	A	N-methyl scopolamine	non-polymer		318.4	1	Chemie (3C0)
3	A	MERCURY (II) ION	non-polymer		200.6	3	Chemie (HG)
4		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 10 - 214 (UniProt: P08172)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Ser 4	-	expression tag
Thr 5	-	expression tag
Asp 6	-	expression tag
Ser 7	-	expression tag
Ser 8	-	expression tag
Asp 9	-	expression tag
Arg 110	Ser 110	engineered mutation

A: 998 - 1106 (PDB: 5YC8)
A: null - 466 (UniProt: P08172)

PDB	External Database	Details
Leu 467	-	expression tag
Glu 468	-	expression tag
Val 469	-	expression tag
Leu 470	-	expression tag
Phe 471	-	expression tag
Gln 472	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47293.2
Non-Polymers*	Number of molecules	4
	Total formula weight	920.2
All*	Total formula weight	48213.4

*Water molecules are not included.