5Y9X

Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with alphaketoglutarate and coblat(II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Uncharacterized protein KdoO	polymer	318	36239.3	1	UniProt (B3DUR4) Pfam (PF11004) In PDB	Methylacidiphilum infernorum (isolate V4)	3-Deoxy-D-manno-oct-2-ulosonic acid hydroxylase
2	A	COBALT (II) ION	non-polymer		58.9	1	Chemie (CO)
3	A	2-OXOGLUTARIC ACID	non-polymer		146.1	1	Chemie (AKG)
4	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	4	Chemie (PG4)
5	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A	ACETATE ION	non-polymer		59.0	6	Chemie (ACT)
7	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
9		water	water		18.0	320	Chemie (HOH)

Sequence modifications

A: 1 - 305 (UniProt: B3DUR4)

PDB	External Database	Details
Lys 306	-	expression tag
Leu 307	-	expression tag
Ala 308	-	expression tag
Ala 309	-	expression tag
Ala 310	-	expression tag
Leu 311	-	expression tag
Glu 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36239.3
Non-Polymers*	Number of molecules	19
	Total formula weight	1822.8
All*	Total formula weight	38062.2

*Water molecules are not included.