5Y9I

Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with Co(II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Uncharacterized protein KdoO	polymer	318	36239.3	1	UniProt (B3DUR4)	Methylacidiphilum infernorum (isolate V4)	3-Deoxy-D-manno-oct-2-ulosonic acid hydroxylase
2	B (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	C (A)	COBALT (II) ION	non-polymer		58.9	1	Chemie (CO)
4	D (A)	octyl beta-D-glucopyranoside	non-polymer		292.4	1	Chemie (BOG)
5	E, F, G (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6	H, I (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
7	J (A)	water	water		18.0	391	Chemie (HOH)

Sequence modifications

A: 1 - 305 (UniProt: B3DUR4)

PDB	External Database	Details
Lys 306	-	expression tag
Leu 307	-	expression tag
Ala 308	-	expression tag
Ala 309	-	expression tag
Ala 310	-	expression tag
Leu 311	-	expression tag
Glu 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36239.3
Non-Polymers*	Number of molecules	8
	Total formula weight	671.8
All*	Total formula weight	36911.1

*Water molecules are not included.