5Y9I

Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with Co(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.263, 59.566, 116.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.875 - 1.901
R-factor	0.1536
Rwork	0.151
R-free	0.19480
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.827
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	51868
<I/σ(I)>	19.2
Completeness [%]	98.3
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1M Sodium acetate (pH=4.6), 200mM Ammonium sulfate, 25% v/v PEG 4000