5Y6L

A subcomplex crystal structure of human cytosolic aspartyl-tRNA synthetase and heterotetrameric glutathione transferase-homology domains in multi-tRNA synthetase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methionine--tRNA ligase, cytoplasmic	polymer	232	25956.4	1	UniProt (P56192) Pfam (PF18485) Pfam (PF00043)	Homo sapiens (Human)	Methionyl-tRNA synthetase,MetRS
2	B (B)	Eukaryotic translation elongation factor 1 epsilon-1	polymer	186	21226.0	1	UniProt (O43324) Pfam (PF21972)	Homo sapiens (Human)	Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3,Elongation factor p18,Multisynthase complex auxiliary component p18
3	C (C)	Bifunctional glutamate/proline--tRNA ligase	polymer	175	19310.7	1	UniProt (P07814) Pfam (PF21972)	Homo sapiens (Human)	Bifunctional aminoacyl-tRNA synthetase,Cell proliferation-inducing gene 32 protein,Glutamatyl-prolyl-tRNA synthetase
4	D (D)	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	polymer	240	26790.9	1	UniProt (Q13155) Pfam (PF18569) Pfam (PF00043)	Homo sapiens (Human)	Multisynthase complex auxiliary component p38,Protein JTV-1
5	E (E)	Aspartate--tRNA ligase, cytoplasmic	polymer	521	59384.4	1	UniProt (P14868) Pfam (PF01336) Pfam (PF00152)	Homo sapiens (Human)	Aspartyl-tRNA synthetase,AspRS,Cell proliferation-inducing gene 40 protein
6	F, G (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	H, I, J, K, L (A, B, C, D, E)	water	water		18.0	78	Chemie (HOH)

Sequence modifications

A: 1 - 224 (UniProt: P56192)

PDB	External Database	Details
Arg 171	Ser 171	engineered mutation
Leu 225	-	expression tag
Glu 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag

B: 1 - 174 (UniProt: O43324)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 147	Cys 147	engineered mutation

C: 1 - 175 (UniProt: P07814)

PDB	External Database	Details
Ser 92	Cys 92	engineered mutation
Ser 105	Cys 105	engineered mutation
Ser 123	Cys 123	engineered mutation

D: 90 - 320 (UniProt: Q13155)

PDB	External Database	Details
Met 89	-	initiating methionine
Leu 321	-	expression tag
Glu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

E: 1 - 501 (UniProt: P14868)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	152668.4
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	152858.4

*Water molecules are not included.