5XYJ

Purification,crystallization and structural analysis of cytoplastic acetoacetyl-CoA thiolase from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Acetyl-CoA acetyltransferase	polymer	408	42904.7	2	UniProt (P41338) Pfam (PF00108) Pfam (PF02803) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Acetoacetyl-CoA thiolase,Ergosterol biosynthesis protein 10
2	B, A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3		water	water		18.0	809	Chemie (HOH)

Sequence modifications

B, A: 1 - 398 (UniProt: P41338)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 91	Cys 91	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85809.4
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	85993.6

*Water molecules are not included.