5XYJ

Purification,crystallization and structural analysis of cytoplastic acetoacetyl-CoA thiolase from Saccharomyces cerevisiae

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
A	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005773	cellular_component	vacuole
A	0005829	cellular_component	cytosol
A	0006696	biological_process	ergosterol biosynthetic process
A	0008299	biological_process	isoprenoid biosynthetic process
A	0010142	biological_process	farnesyl diphosphate biosynthetic process, mevalonate pathway
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0046395	biological_process	carboxylic acid catabolic process
B	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
B	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005773	cellular_component	vacuole
B	0005829	cellular_component	cytosol
B	0006696	biological_process	ergosterol biosynthetic process
B	0008299	biological_process	isoprenoid biosynthetic process
B	0010142	biological_process	farnesyl diphosphate biosynthetic process, mevalonate pathway
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0046395	biological_process	carboxylic acid catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue GOL B 401

Chain	Residue
B	LEU151
B	SER252
B	PHE325
B	HIS354
B	HOH543
B	HOH638
B	HOH777

---

site_id	AC2
Number of Residues	9
Details	binding site for residue GOL A 401

Chain	Residue
A	PHE325
A	HIS354
A	HOH520
A	HOH537
A	HOH558
A	HOH564
A	HOH739
A	LEU151
A	SER252

---

Functional Information from PROSITE/UniProt

site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. GVAAICNGgGgAsS

Chain	Residue	Details
B	GLY379-SER392

---

site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NvyGGaVAlGHPlGcSG

Chain	Residue	Details
B	ASN344-GLY360

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Acyl-thioester intermediate","evidences":[{"source":"UniProtKB","id":"P24752","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10020","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P24752","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---