5XYJ
Purification,crystallization and structural analysis of cytoplastic acetoacetyl-CoA thiolase from Saccharomyces cerevisiae
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005773 | cellular_component | vacuole |
| A | 0005829 | cellular_component | cytosol |
| A | 0006635 | biological_process | fatty acid beta-oxidation |
| A | 0006696 | biological_process | ergosterol biosynthetic process |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005773 | cellular_component | vacuole |
| B | 0005829 | cellular_component | cytosol |
| B | 0006635 | biological_process | fatty acid beta-oxidation |
| B | 0006696 | biological_process | ergosterol biosynthetic process |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue GOL B 401 |
| Chain | Residue |
| B | LEU151 |
| B | SER252 |
| B | PHE325 |
| B | HIS354 |
| B | HOH543 |
| B | HOH638 |
| B | HOH777 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 401 |
| Chain | Residue |
| A | PHE325 |
| A | HIS354 |
| A | HOH520 |
| A | HOH537 |
| A | HOH558 |
| A | HOH564 |
| A | HOH739 |
| A | LEU151 |
| A | SER252 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Acyl-thioester intermediate => ECO:0000250|UniProtKB:P24752 |
| Chain | Residue | Details |
| B | ALA91 | |
| A | ALA91 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10020 |
| Chain | Residue | Details |
| B | HIS354 | |
| B | CYS384 | |
| A | HIS354 | |
| A | CYS384 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P24752 |
| Chain | Residue | Details |
| B | ALA248 | |
| B | ALA249 | |
| B | ALA251 | |
| B | SER252 | |
| B | VAL350 | |
| A | TYR186 | |
| A | LYS231 | |
| A | ALA248 | |
| A | ALA249 | |
| A | ALA251 | |
| A | SER252 | |
| A | VAL350 | |
| B | LYS231 | |
| B | TYR186 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylserine => ECO:0000269|Ref.4, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| B | SER2 | |
| A | SER2 |
