5XV0

Crystal structure of Rib7 mutant D33N from Methanosarcina mazei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Conserved protein	polymer	240	26104.1	6	UniProt (Q8PYN5) Pfam (PF01872) In PDB	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)
2	A, B, C, D, E...	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	6	Chemie (NAP)
3		water	water		18.0	699	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 228 (UniProt: Q8PYN5)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 33	Asp 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	156624.8
Non-Polymers*	Number of molecules	6
	Total formula weight	4460.4
All*	Total formula weight	161085.2

*Water molecules are not included.