5XSD

XylFII-LytSN complex mutant - D103A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Periplasmic binding protein/LacI transcriptional regulator	polymer	306	33660.4	2	UniProt (A6LW07) Pfam (PF13407) In PDB	Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052)
2	L	Signal transduction histidine kinase, LytS	polymer	147	16845.5	1	UniProt (A6LW08) In PDB	Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052)
3		water	water		18.0	140	Chemie (HOH)

Sequence modifications

A, B: 1 - 302 (UniProt: A6LW07)

PDB	External Database	Details
Met -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag
Ala 103	Asp 127	engineered mutation

L: 1 - 134 (UniProt: A6LW08)

PDB	External Database	Details
Met -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	84166.4
All*	Total formula weight	84166.4

*Water molecules are not included.