5XOU

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 7T8 on the guide strand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	TtAgo (D546N)	polymer	685	76727.8	2	UniProt (Q746M7)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	Uncharacterized protein
2	C, E (C, E)	DNA (5'-D(P*TP*GP*AP*GP*GP*TP*AP*TP*GP*GP*TP*TP*GP*T)-3')	polymer	22	6892.5	2		Thermus thermophilus
3	D, F (D, F)	DNA (5'-D(*AP*CP*AP*AP*CP*CP*TP*AP*CP*TP*AP*CP*CP*TP*CP*G)-3')	polymer	19	5708.7	2		Thermus thermophilus
4	G, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J, K, L (A, B, E, F)	water	water		18.0	19	Chemie (HOH)

Sequence modifications

A, B: 1 - 685 (UniProt: Q746M7)

PDB	External Database	Details
Asn 546	Asp 546	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178657.9
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	178706.5

*Water molecules are not included.