5XOU

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 7T8 on the guide strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	CCD
Collection date	2015-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	112.747, 114.982, 160.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.170 - 2.630
R-factor	0.237
Rwork	0.235
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpy
RMSD bond length	0.019
RMSD bond angle	2.442
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.600	2.630
Rmerge		0.570
Number of reflections	60975
<I/σ(I)>	17.7	2
Completeness [%]	97.5	95.3
Redundancy	4.9	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		308	3.0 M NaAc.3H2O, 100 mM Bis-Tris propane, pH 7.0-7.2