5XCA

Crystal structure of GH45 endoglucanase EG27II D137A mutant in complex with cellobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endo-beta-1,4-glucanase	polymer	190	20871.0	1	UniProt (A7KMF0) Pfam (PF22514) In PDB	Ampullaria crossean
2	B	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO)		beta-cellobiose
3		water	water		18.0	175	Chemie (HOH)

Sequence modifications

A: 1 - 179 (UniProt: A7KMF0)

PDB	External Database	Details
Ser -4	-	expression tag
Leu -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Arg 0	-	expression tag
Ala 137	Asp 153	engineered mutation
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	20871.0
Branched	Number of molecules	1
	Total formula weight	342.3
All*	Total formula weight	21213.3

*Water molecules are not included.