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5XCA

Crystal structure of GH45 endoglucanase EG27II D137A mutant in complex with cellobiose

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL26B1
Synchrotron siteSPring-8
BeamlineBL26B1
Temperature [K]100
Detector technologyCCD
Collection date2016-12-03
DetectorRAYONIX MX225HE
Wavelength(s)1.00000
Spacegroup nameP 21 21 21
Unit cell lengths34.321, 46.531, 91.891
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution41.510 - 1.350
R-factor0.156
Rwork0.154
R-free0.19300
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1wc2
RMSD bond length0.014
RMSD bond angle1.277
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwarePHENIX ((1.10.1_2155: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.370
High resolution limit [Å]1.3501.350
Rmerge0.0880.428
Number of reflections32869
<I/σ(I)>33.54
Completeness [%]99.599.9
Redundancy76.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.529325% PEG4000, 200mM KCl, 50mM MES

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