5X3T

VapBC from Mycobacterium tuberculosis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Antitoxin VapB26	polymer	71	7729.6	4	UniProt (O53778) Pfam (PF01402) In PDB	Mycobacterium tuberculosis
2	B, D, F, H	Ribonuclease VapC26	polymer	155	16782.6	4	UniProt (O53779) Pfam (PF01850) In PDB	Mycobacterium tuberculosis	RNase VapC26,Toxin VapC26
3	H	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4		water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 71 (UniProt: O53778)

PDB	External Database	Details
Mse 50	Leu 50	engineered mutation

B, D, F, H: 1 - 135 (UniProt: O53779)

PDB	External Database	Details
Mse -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	98048.6
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	98072.9

*Water molecules are not included.