5X3T
VapBC from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 41 |
Unit cell lengths | 64.221, 64.221, 216.134 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.780 - 2.650 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.23900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 49908 | |
<I/σ(I)> | 70.2 | |
Completeness [%] | 99.8 | |
Redundancy | 24.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Tacsimate |