5X3K

Kfla1895 D451A mutant in complex with isomaltose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glycoside hydrolase family 31	polymer	743	82316.6	2	UniProt (D2PPM7) Pfam (PF21568) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) In PDB	Kribbella flavida DSM 17836
2	C	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G69864PN)
3	D	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G69864PN)
4	A, B	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6		water	water		18.0	576	Chemie (HOH)

Sequence modifications

A, B: 1 - 723 (UniProt: D2PPM7)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 451	Asp 451	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164633.3
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	13
	Total formula weight	1236.9
All*	Total formula weight	166554.8

*Water molecules are not included.