5X3K
Kfla1895 D451A mutant in complex with isomaltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 180.584, 180.584, 392.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.420 - 2.500 |
R-factor | 0.1823 |
Rwork | 0.182 |
R-free | 0.21600 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.957 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.420 |
High resolution limit [Å] | 2.500 |
Number of reflections | 85003 |
<I/σ(I)> | 22.3 |
Completeness [%] | 99.9 |
Redundancy | 20.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.8 M lithium sulfate, 0.1 M imidazole |