5X3J

Kfla1895 D451A mutant in complex with cyclobis-(1->6)-alpha-nigerosyl

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase family 31	polymer	743	82316.6	2	UniProt (D2PPM7) Pfam (PF21568) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365)	Kribbella flavida DSM 17836
2	C, D (C, D)	Cyclic alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		666.6	2	In PDB GlyTouCan (G67006KT)
3	AA, BA, CA, DA, E... (B, A)	SULFATE ION	non-polymer		96.1	20	Chemie (SO4)
4	Q, R, S, T, U... (A)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
5	EA, FA (A, B)	water	water		18.0	592	Chemie (HOH)

Sequence modifications

A, B: 1 - 723 (UniProt: D2PPM7)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 451	Asp 451	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164633.3
Branched	Number of molecules	2
	Total formula weight	1333.2
Non-Polymers*	Number of molecules	26
	Total formula weight	2473.8
All*	Total formula weight	168440.3

*Water molecules are not included.