5X3J

Kfla1895 D451A mutant in complex with cyclobis-(1->6)-alpha-nigerosyl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	180.538, 180.538, 391.626
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.402 - 2.300
R-factor	0.1897
Rwork	0.189
R-free	0.20830
RMSD bond length	0.006
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.400
High resolution limit [Å]	2.300
Number of reflections	108703
<I/σ(I)>	16.17
Completeness [%]	99.9
Redundancy	20.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.8 M lithium sulfate, 0.1 M imidazole