5X2M

Crystal structure of the medaka fish taste receptor T1r2a-T1r3 ligand binding domains in complex with L-glutamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Taste receptor, type 1, member 2a	polymer	461	51471.1	2	UniProt (A0A173M0G2) Pfam (PF01094)	Oryzias latipes (Japanese rice fish)	T1r2a
2	B, D (B, D)	Taste receptor, type 1, member 3	polymer	478	53132.0	2	UniProt (A0A173M094) Pfam (PF01094)	Oryzias latipes (Japanese rice fish)	T1r3
3	E, G (H, J)	Fab16A Heavy chain	polymer	225	24182.2	2		Mus musculus (Mouse)
4	F, H (L, K)	Fab16A Light chain	polymer	217	23899.2	2		Mus musculus (Mouse)
5	AA, BA, CA, DA, EA... (C, A, D, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)
6	HA, P, PA, Y (C, A, D, B)	GLUTAMINE	non-polymer		146.1	4	Chemie (GLN)
7	IA, Q, QA (C, A, D)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
8	RA, Z (D, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
9	SA (L)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
10	AB, TA, UA, VA, WA... (K, A, B, C, D...)	water	water		18.0	775	Chemie (HOH)

Sequence modifications

A, C: 20 - 474 (UniProt: A0A173M0G2)

PDB	External Database	Details
Ser 475	-	expression tag
Gly 476	-	expression tag
Ile 477	-	expression tag
Glu 478	-	expression tag
Gly 479	-	expression tag
Arg 480	-	expression tag

B, D: 20 - 491 (UniProt: A0A173M094)

PDB	External Database	Details
Ser 492	-	expression tag
Gly 493	-	expression tag
Ile 494	-	expression tag
Glu 495	-	expression tag
Gly 496	-	expression tag
Arg 497	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	305369.1
Non-Polymers*	Number of molecules	37
	Total formula weight	6737.1
All*	Total formula weight	312106.2

*Water molecules are not included.