5X2M

Crystal structure of the medaka fish taste receptor T1r2a-T1r3 ligand binding domains in complex with L-glutamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	99.656, 116.355, 129.476
Unit cell angles	90.00, 92.20, 90.00

Refinement procedure

Resolution	49.891 - 2.206
R-factor	0.1758
Rwork	0.173
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e4u 1a6t
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	142788	7093
<I/σ(I)>	15.6	2.1
Completeness [%]	97.1	96.2
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	12~13% (w/v) PEG 1500, 3% (v/v) PEG400, 0.1 M MES, 5 mM Glutamine