5WSG
Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 3 | C (J) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 21 |
| 4 | D (O) | Pre-mRNA-splicing factor PRP46 | polymer | 451 | 50771.3 | 1 | UniProt (Q12417) Pfam (PF00400) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46 |
| 5 | E (P) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 6 | F (Q) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Extracellular mutant protein 2,Synthetic lethality with U2 protein 11 |
| 7 | G (R) | Pre-mRNA-splicing factor CWC2 | polymer | 339 | 38486.6 | 1 | UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3 |
| 8 | H (S) | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 15 |
| 9 | I (T) | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Bud site selection protein 31,Complexed with CEF1 protein 14 |
| 10 | J (Z) | Pre-mRNA-splicing factor CWC22 | polymer | 577 | 67386.1 | 1 | UniProt (P53333) Pfam (PF02847) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 22 |
| 11 | K (B) | 5'-exon | polymer | 13 | 4112.5 | 1 | Saccharomyces cerevisiae S288c | ||
| 12 | L (N) | 5'-intron-lariat | polymer | 15 | 4694.7 | 1 | Saccharomyces cerevisiae S288c | ||
| 13 | M (D) | U5 snRNA | polymer | 214 | 68643.3 | 1 | Saccharomyces cerevisiae S288c | ||
| 14 | N (E) | Saccharomyces cerevisiae S288c SNR6 snRNA | polymer | 112 | 35883.2 | 1 | REF (831416131) | Saccharomyces cerevisiae S288c | |
| 15 | O (L) | RNA (91-MER) | polymer | 1175 | 376267.4 | 1 | Saccharomyces cerevisiae S288c | ||
| 16 | P (M) | 3'-intron-lariat | polymer | 23 | 7301.4 | 1 | Saccharomyces cerevisiae S288c | ||
| 17 | Q (c) | Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 | polymer | 579 | 60160.5 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
| 18 | R (d) | Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 | polymer | 652 | 64183.4 | 1 | UniProt (Q12309) Pfam (PF23233) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3 |
| 19 | S (I) | Pre-mRNA-splicing factor SYF2 | polymer | 215 | 24850.7 | 1 | UniProt (P53277) Pfam (PF08231) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2 |
| 20 | T (v) | Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 | polymer | 858 | 78040.5 | 1 | UniProt (Q04048) Pfam (PF23231) | Saccharomyces cerevisiae S288c | PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1 |
| 21 | U (n) | Pre-mRNA-processing factor 17 | polymer | 455 | 52128.8 | 1 | UniProt (P40968) Pfam (PF00400) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Cell division control protein 40 |
| 22 | V, W, X, Y (o, p, q, r) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (P32523) Pfam (PF08606) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 23 | Z (t) | Pre-mRNA-splicing factor SNT309 | polymer | 175 | 20741.5 | 1 | UniProt (Q06091) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309 |
| 24 | AA, HA (k, F) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 25 | BA, IA (i, G) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 26 | CA, JA (h, H) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 27 | DA, KA (j, K) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 28 | EA, LA (l, U) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 29 | FA, MA (m, V) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 30 | GA, NA (g, W) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 31 | OA (X) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | U2 snRNP B'' |
| 32 | PA (Y) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 33 | QA (b) | 3'-exon-intron | polymer | 14 | 4349.5 | 1 | Saccharomyces cerevisiae S288c | ||
| 34 | RA (e) | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 | polymer | 1071 | 121815.2 | 1 | UniProt (P15938) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 35 | SA (f) | Pre-mRNA-splicing factor 18 | polymer | 251 | 28414.4 | 1 | UniProt (P33411) Pfam (PF02840) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 36 | TA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 37 | BB, CB, DB, EB, FB... (B, E, C) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 38 | AB, VA, WA, XA, YA... (T, Q, R) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
c: 9 - 253 (UniProt: Q03654)
c: 254 - 481 (PDB: 5WSG)
c: 482 - 587 (UniProt: Q03654)
d: 36 - 275 (UniProt: Q12309)
d: 276 - 687 (PDB: 5WSG)
v: 1 - 568 (PDB: 5WSG)
v: 569 - 734 (UniProt: Q04048)
v: 735 - 858 (PDB: 5WSG)
c: 254 - 481 (PDB: 5WSG)
c: 482 - 587 (UniProt: Q03654)
d: 36 - 275 (UniProt: Q12309)
d: 276 - 687 (PDB: 5WSG)
v: 1 - 568 (PDB: 5WSG)
v: 569 - 734 (UniProt: Q04048)
v: 735 - 858 (PDB: 5WSG)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 45 |
| Total formula weight | 2089436.9 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1061.5 | |
| All* | Total formula weight | 2090498.4 |
