5WSG

Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000244	biological_process	spliceosomal tri-snRNP complex assembly
A	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
A	0000386	molecular_function	second spliceosomal transesterification activity
A	0000387	biological_process	spliceosomal snRNP assembly
A	0000389	biological_process	mRNA 3'-splice site recognition
A	0000393	biological_process	spliceosomal conformational changes to generate catalytic conformation
A	0000395	biological_process	mRNA 5'-splice site recognition
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0000974	cellular_component	Prp19 complex
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005681	cellular_component	spliceosomal complex
A	0005682	cellular_component	U5 snRNP
A	0005737	cellular_component	cytoplasm
A	0006397	biological_process	mRNA processing
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008380	biological_process	RNA splicing
A	0017070	molecular_function	U6 snRNA binding
A	0030619	molecular_function	U1 snRNA binding
A	0030620	molecular_function	U2 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
A	0045292	biological_process	mRNA cis splicing, via spliceosome
A	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0097157	molecular_function	pre-mRNA intronic binding
A	1990904	cellular_component	ribonucleoprotein complex
c	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
c	0000386	molecular_function	second spliceosomal transesterification activity
c	0000398	biological_process	mRNA splicing, via spliceosome
c	0000974	cellular_component	Prp19 complex
c	0003677	molecular_function	DNA binding
c	0005515	molecular_function	protein binding
c	0005634	cellular_component	nucleus
c	0005681	cellular_component	spliceosomal complex
c	0005737	cellular_component	cytoplasm
c	0006397	biological_process	mRNA processing
c	0008380	biological_process	RNA splicing
c	0071006	cellular_component	U2-type catalytic step 1 spliceosome
C	0000166	molecular_function	nucleotide binding
C	0000244	biological_process	spliceosomal tri-snRNP complex assembly
C	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
C	0000387	biological_process	spliceosomal snRNP assembly
C	0000388	biological_process	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0000974	cellular_component	Prp19 complex
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005682	cellular_component	U5 snRNP
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006397	biological_process	mRNA processing
C	0008380	biological_process	RNA splicing
C	0030623	molecular_function	U5 snRNA binding
C	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
C	0071007	cellular_component	U2-type catalytic step 2 spliceosome
d	0000398	biological_process	mRNA splicing, via spliceosome
d	0006396	biological_process	RNA processing
e	0000166	molecular_function	nucleotide binding
e	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
e	0000378	biological_process	RNA exon ligation
e	0000386	molecular_function	second spliceosomal transesterification activity
e	0000398	biological_process	mRNA splicing, via spliceosome
e	0003676	molecular_function	nucleic acid binding
e	0003723	molecular_function	RNA binding
e	0003724	molecular_function	RNA helicase activity
e	0004386	molecular_function	helicase activity
e	0005515	molecular_function	protein binding
e	0005524	molecular_function	ATP binding
e	0005634	cellular_component	nucleus
e	0005681	cellular_component	spliceosomal complex
e	0006397	biological_process	mRNA processing
e	0008186	molecular_function	ATP-dependent activity, acting on RNA
e	0008380	biological_process	RNA splicing
e	0016073	biological_process	snRNA metabolic process
e	0016787	molecular_function	hydrolase activity
e	0016887	molecular_function	ATP hydrolysis activity
e	0022613	biological_process	ribonucleoprotein complex biogenesis
e	0034458	molecular_function	3'-5' RNA helicase activity
e	0040031	biological_process	snRNA modification
e	0071007	cellular_component	U2-type catalytic step 2 spliceosome
e	0071826	biological_process	protein-RNA complex organization
f	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
f	0000386	molecular_function	second spliceosomal transesterification activity
f	0000398	biological_process	mRNA splicing, via spliceosome
f	0005634	cellular_component	nucleus
f	0005681	cellular_component	spliceosomal complex
f	0005682	cellular_component	U5 snRNP
f	0006397	biological_process	mRNA processing
f	0008380	biological_process	RNA splicing
f	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
f	0071021	cellular_component	U2-type post-spliceosomal complex
f	0071028	biological_process	nuclear mRNA surveillance
F	0000245	biological_process	spliceosomal complex assembly
F	0000387	biological_process	spliceosomal snRNP assembly
F	0000395	biological_process	mRNA 5'-splice site recognition
F	0000398	biological_process	mRNA splicing, via spliceosome
F	0003674	molecular_function	molecular_function
F	0003723	molecular_function	RNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005682	cellular_component	U5 snRNP
F	0005685	cellular_component	U1 snRNP
F	0005686	cellular_component	U2 snRNP
F	0005687	cellular_component	U4 snRNP
F	0005737	cellular_component	cytoplasm
F	0006397	biological_process	mRNA processing
F	0008380	biological_process	RNA splicing
F	0032991	cellular_component	protein-containing complex
F	0036261	biological_process	7-methylguanosine cap hypermethylation
F	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
F	0070990	molecular_function	snRNP binding
F	0071004	cellular_component	U2-type prespliceosome
F	0071013	cellular_component	catalytic step 2 spliceosome
F	1903241	biological_process	U2-type prespliceosome assembly
F	1990904	cellular_component	ribonucleoprotein complex
g	0000245	biological_process	spliceosomal complex assembly
g	0000387	biological_process	spliceosomal snRNP assembly
g	0000395	biological_process	mRNA 5'-splice site recognition
g	0000398	biological_process	mRNA splicing, via spliceosome
g	0000974	cellular_component	Prp19 complex
g	0003674	molecular_function	molecular_function
g	0003723	molecular_function	RNA binding
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0005681	cellular_component	spliceosomal complex
g	0005682	cellular_component	U5 snRNP
g	0005685	cellular_component	U1 snRNP
g	0005686	cellular_component	U2 snRNP
g	0005737	cellular_component	cytoplasm
g	0005829	cellular_component	cytosol
g	0006397	biological_process	mRNA processing
g	0008150	biological_process	biological_process
g	0008380	biological_process	RNA splicing
g	0030532	cellular_component	small nuclear ribonucleoprotein complex
g	0034715	cellular_component	pICln-Sm protein complex
g	0036261	biological_process	7-methylguanosine cap hypermethylation
g	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
g	0071004	cellular_component	U2-type prespliceosome
g	0071011	cellular_component	precatalytic spliceosome
g	0071013	cellular_component	catalytic step 2 spliceosome
g	1903241	biological_process	U2-type prespliceosome assembly
g	1990904	cellular_component	ribonucleoprotein complex
G	0000245	biological_process	spliceosomal complex assembly
G	0000387	biological_process	spliceosomal snRNP assembly
G	0000395	biological_process	mRNA 5'-splice site recognition
G	0000398	biological_process	mRNA splicing, via spliceosome
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005681	cellular_component	spliceosomal complex
G	0005682	cellular_component	U5 snRNP
G	0005685	cellular_component	U1 snRNP
G	0005686	cellular_component	U2 snRNP
G	0005687	cellular_component	U4 snRNP
G	0005737	cellular_component	cytoplasm
G	0006397	biological_process	mRNA processing
G	0008380	biological_process	RNA splicing
G	0032991	cellular_component	protein-containing complex
G	0034715	cellular_component	pICln-Sm protein complex
G	0036261	biological_process	7-methylguanosine cap hypermethylation
G	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
G	0071004	cellular_component	U2-type prespliceosome
G	0071011	cellular_component	precatalytic spliceosome
G	1903241	biological_process	U2-type prespliceosome assembly
G	1990904	cellular_component	ribonucleoprotein complex
G	1990935	molecular_function	splicing factor binding
h	0000245	biological_process	spliceosomal complex assembly
h	0000387	biological_process	spliceosomal snRNP assembly
h	0000395	biological_process	mRNA 5'-splice site recognition
h	0000398	biological_process	mRNA splicing, via spliceosome
h	0003723	molecular_function	RNA binding
h	0005515	molecular_function	protein binding
h	0005634	cellular_component	nucleus
h	0005681	cellular_component	spliceosomal complex
h	0005682	cellular_component	U5 snRNP
h	0005685	cellular_component	U1 snRNP
h	0005686	cellular_component	U2 snRNP
h	0005737	cellular_component	cytoplasm
h	0006397	biological_process	mRNA processing
h	0008266	molecular_function	poly(U) RNA binding
h	0008380	biological_process	RNA splicing
h	0034715	cellular_component	pICln-Sm protein complex
h	0036261	biological_process	7-methylguanosine cap hypermethylation
h	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
h	0071013	cellular_component	catalytic step 2 spliceosome
h	0120114	cellular_component	Sm-like protein family complex
h	1903241	biological_process	U2-type prespliceosome assembly
h	1990904	cellular_component	ribonucleoprotein complex
h	1990935	molecular_function	splicing factor binding
H	0000245	biological_process	spliceosomal complex assembly
H	0000387	biological_process	spliceosomal snRNP assembly
H	0000395	biological_process	mRNA 5'-splice site recognition
H	0000398	biological_process	mRNA splicing, via spliceosome
H	0003723	molecular_function	RNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005681	cellular_component	spliceosomal complex
H	0005682	cellular_component	U5 snRNP
H	0005685	cellular_component	U1 snRNP
H	0005686	cellular_component	U2 snRNP
H	0005737	cellular_component	cytoplasm
H	0006397	biological_process	mRNA processing
H	0008266	molecular_function	poly(U) RNA binding
H	0008380	biological_process	RNA splicing
H	0034715	cellular_component	pICln-Sm protein complex
H	0036261	biological_process	7-methylguanosine cap hypermethylation
H	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
H	0071013	cellular_component	catalytic step 2 spliceosome
H	0120114	cellular_component	Sm-like protein family complex
H	1903241	biological_process	U2-type prespliceosome assembly
H	1990904	cellular_component	ribonucleoprotein complex
H	1990935	molecular_function	splicing factor binding
i	0000245	biological_process	spliceosomal complex assembly
i	0000387	biological_process	spliceosomal snRNP assembly
i	0000395	biological_process	mRNA 5'-splice site recognition
i	0000398	biological_process	mRNA splicing, via spliceosome
i	0003723	molecular_function	RNA binding
i	0005515	molecular_function	protein binding
i	0005634	cellular_component	nucleus
i	0005681	cellular_component	spliceosomal complex
i	0005682	cellular_component	U5 snRNP
i	0005685	cellular_component	U1 snRNP
i	0005686	cellular_component	U2 snRNP
i	0005687	cellular_component	U4 snRNP
i	0005737	cellular_component	cytoplasm
i	0006397	biological_process	mRNA processing
i	0008380	biological_process	RNA splicing
i	0032991	cellular_component	protein-containing complex
i	0034715	cellular_component	pICln-Sm protein complex
i	0036261	biological_process	7-methylguanosine cap hypermethylation
i	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
i	0071004	cellular_component	U2-type prespliceosome
i	0071011	cellular_component	precatalytic spliceosome
i	1903241	biological_process	U2-type prespliceosome assembly
i	1990904	cellular_component	ribonucleoprotein complex
i	1990935	molecular_function	splicing factor binding
I	0000384	molecular_function	first spliceosomal transesterification activity
I	0000386	molecular_function	second spliceosomal transesterification activity
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0000974	cellular_component	Prp19 complex
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005681	cellular_component	spliceosomal complex
I	0005829	cellular_component	cytosol
I	0006397	biological_process	mRNA processing
I	0008380	biological_process	RNA splicing
I	0071004	cellular_component	U2-type prespliceosome
I	0071006	cellular_component	U2-type catalytic step 1 spliceosome
I	0071007	cellular_component	U2-type catalytic step 2 spliceosome
I	0071008	cellular_component	U2-type post-mRNA release spliceosomal complex
j	0000245	biological_process	spliceosomal complex assembly
j	0000387	biological_process	spliceosomal snRNP assembly
j	0000395	biological_process	mRNA 5'-splice site recognition
j	0000398	biological_process	mRNA splicing, via spliceosome
j	0003674	molecular_function	molecular_function
j	0003723	molecular_function	RNA binding
j	0005515	molecular_function	protein binding
j	0005634	cellular_component	nucleus
j	0005681	cellular_component	spliceosomal complex
j	0005682	cellular_component	U5 snRNP
j	0005685	cellular_component	U1 snRNP
j	0005686	cellular_component	U2 snRNP
j	0005687	cellular_component	U4 snRNP
j	0005737	cellular_component	cytoplasm
j	0006397	biological_process	mRNA processing
j	0008150	biological_process	biological_process
j	0008380	biological_process	RNA splicing
j	0032991	cellular_component	protein-containing complex
j	0034719	cellular_component	SMN-Sm protein complex
j	0036261	biological_process	7-methylguanosine cap hypermethylation
j	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
j	0071004	cellular_component	U2-type prespliceosome
j	0071011	cellular_component	precatalytic spliceosome
j	0071013	cellular_component	catalytic step 2 spliceosome
j	0097526	cellular_component	spliceosomal tri-snRNP complex
j	1903241	biological_process	U2-type prespliceosome assembly
j	1990904	cellular_component	ribonucleoprotein complex
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0003674	molecular_function	molecular_function
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005681	cellular_component	spliceosomal complex
J	0005684	cellular_component	U2-type spliceosomal complex
J	0005737	cellular_component	cytoplasm
J	0006397	biological_process	mRNA processing
J	0008380	biological_process	RNA splicing
k	0000245	biological_process	spliceosomal complex assembly
k	0000387	biological_process	spliceosomal snRNP assembly
k	0000395	biological_process	mRNA 5'-splice site recognition
k	0000398	biological_process	mRNA splicing, via spliceosome
k	0003674	molecular_function	molecular_function
k	0003723	molecular_function	RNA binding
k	0005515	molecular_function	protein binding
k	0005634	cellular_component	nucleus
k	0005682	cellular_component	U5 snRNP
k	0005685	cellular_component	U1 snRNP
k	0005686	cellular_component	U2 snRNP
k	0005687	cellular_component	U4 snRNP
k	0005737	cellular_component	cytoplasm
k	0006397	biological_process	mRNA processing
k	0008380	biological_process	RNA splicing
k	0032991	cellular_component	protein-containing complex
k	0036261	biological_process	7-methylguanosine cap hypermethylation
k	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
k	0070990	molecular_function	snRNP binding
k	0071004	cellular_component	U2-type prespliceosome
k	0071013	cellular_component	catalytic step 2 spliceosome
k	1903241	biological_process	U2-type prespliceosome assembly
k	1990904	cellular_component	ribonucleoprotein complex
K	0000245	biological_process	spliceosomal complex assembly
K	0000387	biological_process	spliceosomal snRNP assembly
K	0000395	biological_process	mRNA 5'-splice site recognition
K	0000398	biological_process	mRNA splicing, via spliceosome
K	0003674	molecular_function	molecular_function
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005681	cellular_component	spliceosomal complex
K	0005682	cellular_component	U5 snRNP
K	0005685	cellular_component	U1 snRNP
K	0005686	cellular_component	U2 snRNP
K	0005687	cellular_component	U4 snRNP
K	0005737	cellular_component	cytoplasm
K	0006397	biological_process	mRNA processing
K	0008150	biological_process	biological_process
K	0008380	biological_process	RNA splicing
K	0032991	cellular_component	protein-containing complex
K	0034719	cellular_component	SMN-Sm protein complex
K	0036261	biological_process	7-methylguanosine cap hypermethylation
K	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
K	0071004	cellular_component	U2-type prespliceosome
K	0071011	cellular_component	precatalytic spliceosome
K	0071013	cellular_component	catalytic step 2 spliceosome
K	0097526	cellular_component	spliceosomal tri-snRNP complex
K	1903241	biological_process	U2-type prespliceosome assembly
K	1990904	cellular_component	ribonucleoprotein complex
l	0000243	cellular_component	commitment complex
l	0000245	biological_process	spliceosomal complex assembly
l	0000387	biological_process	spliceosomal snRNP assembly
l	0000395	biological_process	mRNA 5'-splice site recognition
l	0000398	biological_process	mRNA splicing, via spliceosome
l	0003723	molecular_function	RNA binding
l	0003729	molecular_function	mRNA binding
l	0005515	molecular_function	protein binding
l	0005634	cellular_component	nucleus
l	0005681	cellular_component	spliceosomal complex
l	0005682	cellular_component	U5 snRNP
l	0005685	cellular_component	U1 snRNP
l	0005686	cellular_component	U2 snRNP
l	0005687	cellular_component	U4 snRNP
l	0005737	cellular_component	cytoplasm
l	0005829	cellular_component	cytosol
l	0006396	biological_process	RNA processing
l	0006397	biological_process	mRNA processing
l	0008380	biological_process	RNA splicing
l	0034719	cellular_component	SMN-Sm protein complex
l	0036261	biological_process	7-methylguanosine cap hypermethylation
l	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
l	0071004	cellular_component	U2-type prespliceosome
l	0071011	cellular_component	precatalytic spliceosome
l	0071013	cellular_component	catalytic step 2 spliceosome
l	0097526	cellular_component	spliceosomal tri-snRNP complex
l	1903241	biological_process	U2-type prespliceosome assembly
l	1990904	cellular_component	ribonucleoprotein complex
m	0000243	cellular_component	commitment complex
m	0000245	biological_process	spliceosomal complex assembly
m	0000387	biological_process	spliceosomal snRNP assembly
m	0000395	biological_process	mRNA 5'-splice site recognition
m	0000398	biological_process	mRNA splicing, via spliceosome
m	0003723	molecular_function	RNA binding
m	0003729	molecular_function	mRNA binding
m	0005515	molecular_function	protein binding
m	0005634	cellular_component	nucleus
m	0005682	cellular_component	U5 snRNP
m	0005685	cellular_component	U1 snRNP
m	0005686	cellular_component	U2 snRNP
m	0005687	cellular_component	U4 snRNP
m	0005737	cellular_component	cytoplasm
m	0005829	cellular_component	cytosol
m	0006396	biological_process	RNA processing
m	0006397	biological_process	mRNA processing
m	0008380	biological_process	RNA splicing
m	0030532	cellular_component	small nuclear ribonucleoprotein complex
m	0034715	cellular_component	pICln-Sm protein complex
m	0034719	cellular_component	SMN-Sm protein complex
m	0036261	biological_process	7-methylguanosine cap hypermethylation
m	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
m	0071004	cellular_component	U2-type prespliceosome
m	0071011	cellular_component	precatalytic spliceosome
m	0071013	cellular_component	catalytic step 2 spliceosome
m	0097525	cellular_component	spliceosomal snRNP complex
m	0097526	cellular_component	spliceosomal tri-snRNP complex
m	1903241	biological_process	U2-type prespliceosome assembly
m	1990904	cellular_component	ribonucleoprotein complex
n	0000348	biological_process	mRNA branch site recognition
n	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
n	0000386	molecular_function	second spliceosomal transesterification activity
n	0000389	biological_process	mRNA 3'-splice site recognition
n	0000398	biological_process	mRNA splicing, via spliceosome
n	0003729	molecular_function	mRNA binding
n	0005515	molecular_function	protein binding
n	0005634	cellular_component	nucleus
n	0005681	cellular_component	spliceosomal complex
n	0006397	biological_process	mRNA processing
n	0008380	biological_process	RNA splicing
n	0034399	cellular_component	nuclear periphery
n	0042802	molecular_function	identical protein binding
n	0071013	cellular_component	catalytic step 2 spliceosome
o	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
o	0000398	biological_process	mRNA splicing, via spliceosome
o	0000974	cellular_component	Prp19 complex
o	0004842	molecular_function	ubiquitin-protein transferase activity
o	0005515	molecular_function	protein binding
o	0005634	cellular_component	nucleus
o	0005681	cellular_component	spliceosomal complex
o	0005737	cellular_component	cytoplasm
o	0005739	cellular_component	mitochondrion
o	0005829	cellular_component	cytosol
o	0006281	biological_process	DNA repair
o	0006397	biological_process	mRNA processing
o	0006974	biological_process	DNA damage response
o	0008380	biological_process	RNA splicing
o	0016567	biological_process	protein ubiquitination
o	0016740	molecular_function	transferase activity
o	0042802	molecular_function	identical protein binding
o	0061630	molecular_function	ubiquitin protein ligase activity
o	0070534	biological_process	protein K63-linked ubiquitination
o	0071006	cellular_component	U2-type catalytic step 1 spliceosome
O	0000398	biological_process	mRNA splicing, via spliceosome
O	0000974	cellular_component	Prp19 complex
O	0003674	molecular_function	molecular_function
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005681	cellular_component	spliceosomal complex
O	0005737	cellular_component	cytoplasm
O	0006397	biological_process	mRNA processing
O	0008380	biological_process	RNA splicing
O	0071013	cellular_component	catalytic step 2 spliceosome
p	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
p	0000398	biological_process	mRNA splicing, via spliceosome
p	0000974	cellular_component	Prp19 complex
p	0004842	molecular_function	ubiquitin-protein transferase activity
p	0005515	molecular_function	protein binding
p	0005634	cellular_component	nucleus
p	0005681	cellular_component	spliceosomal complex
p	0005737	cellular_component	cytoplasm
p	0005739	cellular_component	mitochondrion
p	0005829	cellular_component	cytosol
p	0006281	biological_process	DNA repair
p	0006397	biological_process	mRNA processing
p	0006974	biological_process	DNA damage response
p	0008380	biological_process	RNA splicing
p	0016567	biological_process	protein ubiquitination
p	0016740	molecular_function	transferase activity
p	0042802	molecular_function	identical protein binding
p	0061630	molecular_function	ubiquitin protein ligase activity
p	0070534	biological_process	protein K63-linked ubiquitination
p	0071006	cellular_component	U2-type catalytic step 1 spliceosome
P	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
P	0000384	molecular_function	first spliceosomal transesterification activity
P	0000386	molecular_function	second spliceosomal transesterification activity
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005681	cellular_component	spliceosomal complex
P	0006397	biological_process	mRNA processing
P	0008380	biological_process	RNA splicing
P	0071006	cellular_component	U2-type catalytic step 1 spliceosome
P	0071007	cellular_component	U2-type catalytic step 2 spliceosome
P	0071014	cellular_component	post-mRNA release spliceosomal complex
q	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0000974	cellular_component	Prp19 complex
q	0004842	molecular_function	ubiquitin-protein transferase activity
q	0005515	molecular_function	protein binding
q	0005634	cellular_component	nucleus
q	0005681	cellular_component	spliceosomal complex
q	0005737	cellular_component	cytoplasm
q	0005739	cellular_component	mitochondrion
q	0005829	cellular_component	cytosol
q	0006281	biological_process	DNA repair
q	0006397	biological_process	mRNA processing
q	0006974	biological_process	DNA damage response
q	0008380	biological_process	RNA splicing
q	0016567	biological_process	protein ubiquitination
q	0016740	molecular_function	transferase activity
q	0042802	molecular_function	identical protein binding
q	0061630	molecular_function	ubiquitin protein ligase activity
q	0070534	biological_process	protein K63-linked ubiquitination
q	0071006	cellular_component	U2-type catalytic step 1 spliceosome
Q	0000398	biological_process	mRNA splicing, via spliceosome
Q	0000974	cellular_component	Prp19 complex
Q	0003723	molecular_function	RNA binding
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005681	cellular_component	spliceosomal complex
Q	0006397	biological_process	mRNA processing
Q	0008380	biological_process	RNA splicing
Q	0017070	molecular_function	U6 snRNA binding
Q	0036002	molecular_function	pre-mRNA binding
Q	0071006	cellular_component	U2-type catalytic step 1 spliceosome
Q	0071007	cellular_component	U2-type catalytic step 2 spliceosome
r	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0000974	cellular_component	Prp19 complex
r	0004842	molecular_function	ubiquitin-protein transferase activity
r	0005515	molecular_function	protein binding
r	0005634	cellular_component	nucleus
r	0005681	cellular_component	spliceosomal complex
r	0005737	cellular_component	cytoplasm
r	0005739	cellular_component	mitochondrion
r	0005829	cellular_component	cytosol
r	0006281	biological_process	DNA repair
r	0006397	biological_process	mRNA processing
r	0006974	biological_process	DNA damage response
r	0008380	biological_process	RNA splicing
r	0016567	biological_process	protein ubiquitination
r	0016740	molecular_function	transferase activity
r	0042802	molecular_function	identical protein binding
r	0061630	molecular_function	ubiquitin protein ligase activity
r	0070534	biological_process	protein K63-linked ubiquitination
r	0071006	cellular_component	U2-type catalytic step 1 spliceosome
R	0000387	biological_process	spliceosomal snRNP assembly
R	0000398	biological_process	mRNA splicing, via spliceosome
R	0000974	cellular_component	Prp19 complex
R	0003676	molecular_function	nucleic acid binding
R	0003723	molecular_function	RNA binding
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005681	cellular_component	spliceosomal complex
R	0006397	biological_process	mRNA processing
R	0008270	molecular_function	zinc ion binding
R	0008380	biological_process	RNA splicing
R	0017070	molecular_function	U6 snRNA binding
R	0033120	biological_process	positive regulation of RNA splicing
R	0036002	molecular_function	pre-mRNA binding
R	0045292	biological_process	mRNA cis splicing, via spliceosome
R	0045787	biological_process	positive regulation of cell cycle
R	0046872	molecular_function	metal ion binding
R	0071006	cellular_component	U2-type catalytic step 1 spliceosome
R	0071007	cellular_component	U2-type catalytic step 2 spliceosome
S	0000398	biological_process	mRNA splicing, via spliceosome
S	0003674	molecular_function	molecular_function
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005681	cellular_component	spliceosomal complex
S	0005684	cellular_component	U2-type spliceosomal complex
S	0006397	biological_process	mRNA processing
S	0008380	biological_process	RNA splicing
S	0045292	biological_process	mRNA cis splicing, via spliceosome
S	0071013	cellular_component	catalytic step 2 spliceosome
t	0000384	molecular_function	first spliceosomal transesterification activity
t	0000398	biological_process	mRNA splicing, via spliceosome
t	0000974	cellular_component	Prp19 complex
t	0005515	molecular_function	protein binding
t	0005634	cellular_component	nucleus
t	0005681	cellular_component	spliceosomal complex
t	0006397	biological_process	mRNA processing
t	0008380	biological_process	RNA splicing
t	0071006	cellular_component	U2-type catalytic step 1 spliceosome
T	0000282	biological_process	cellular bud site selection
T	0000398	biological_process	mRNA splicing, via spliceosome
T	0003674	molecular_function	molecular_function
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005681	cellular_component	spliceosomal complex
T	0005686	cellular_component	U2 snRNP
T	0006397	biological_process	mRNA processing
T	0008380	biological_process	RNA splicing
U	0000243	cellular_component	commitment complex
U	0000245	biological_process	spliceosomal complex assembly
U	0000387	biological_process	spliceosomal snRNP assembly
U	0000395	biological_process	mRNA 5'-splice site recognition
U	0000398	biological_process	mRNA splicing, via spliceosome
U	0003723	molecular_function	RNA binding
U	0003729	molecular_function	mRNA binding
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005681	cellular_component	spliceosomal complex
U	0005682	cellular_component	U5 snRNP
U	0005685	cellular_component	U1 snRNP
U	0005686	cellular_component	U2 snRNP
U	0005687	cellular_component	U4 snRNP
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0006396	biological_process	RNA processing
U	0006397	biological_process	mRNA processing
U	0008380	biological_process	RNA splicing
U	0034719	cellular_component	SMN-Sm protein complex
U	0036261	biological_process	7-methylguanosine cap hypermethylation
U	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
U	0071004	cellular_component	U2-type prespliceosome
U	0071011	cellular_component	precatalytic spliceosome
U	0071013	cellular_component	catalytic step 2 spliceosome
U	0097526	cellular_component	spliceosomal tri-snRNP complex
U	1903241	biological_process	U2-type prespliceosome assembly
U	1990904	cellular_component	ribonucleoprotein complex
v	0000398	biological_process	mRNA splicing, via spliceosome
v	0006396	biological_process	RNA processing
V	0000243	cellular_component	commitment complex
V	0000245	biological_process	spliceosomal complex assembly
V	0000387	biological_process	spliceosomal snRNP assembly
V	0000395	biological_process	mRNA 5'-splice site recognition
V	0000398	biological_process	mRNA splicing, via spliceosome
V	0003723	molecular_function	RNA binding
V	0003729	molecular_function	mRNA binding
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005682	cellular_component	U5 snRNP
V	0005685	cellular_component	U1 snRNP
V	0005686	cellular_component	U2 snRNP
V	0005687	cellular_component	U4 snRNP
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0006396	biological_process	RNA processing
V	0006397	biological_process	mRNA processing
V	0008380	biological_process	RNA splicing
V	0030532	cellular_component	small nuclear ribonucleoprotein complex
V	0034715	cellular_component	pICln-Sm protein complex
V	0034719	cellular_component	SMN-Sm protein complex
V	0036261	biological_process	7-methylguanosine cap hypermethylation
V	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
V	0071004	cellular_component	U2-type prespliceosome
V	0071011	cellular_component	precatalytic spliceosome
V	0071013	cellular_component	catalytic step 2 spliceosome
V	0097525	cellular_component	spliceosomal snRNP complex
V	0097526	cellular_component	spliceosomal tri-snRNP complex
V	1903241	biological_process	U2-type prespliceosome assembly
V	1990904	cellular_component	ribonucleoprotein complex
W	0000245	biological_process	spliceosomal complex assembly
W	0000387	biological_process	spliceosomal snRNP assembly
W	0000395	biological_process	mRNA 5'-splice site recognition
W	0000398	biological_process	mRNA splicing, via spliceosome
W	0000974	cellular_component	Prp19 complex
W	0003674	molecular_function	molecular_function
W	0003723	molecular_function	RNA binding
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005681	cellular_component	spliceosomal complex
W	0005682	cellular_component	U5 snRNP
W	0005685	cellular_component	U1 snRNP
W	0005686	cellular_component	U2 snRNP
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0006397	biological_process	mRNA processing
W	0008150	biological_process	biological_process
W	0008380	biological_process	RNA splicing
W	0030532	cellular_component	small nuclear ribonucleoprotein complex
W	0034715	cellular_component	pICln-Sm protein complex
W	0036261	biological_process	7-methylguanosine cap hypermethylation
W	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
W	0071004	cellular_component	U2-type prespliceosome
W	0071011	cellular_component	precatalytic spliceosome
W	0071013	cellular_component	catalytic step 2 spliceosome
W	1903241	biological_process	U2-type prespliceosome assembly
W	1990904	cellular_component	ribonucleoprotein complex
X	0000398	biological_process	mRNA splicing, via spliceosome
X	0003676	molecular_function	nucleic acid binding
X	0003723	molecular_function	RNA binding
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0006397	biological_process	mRNA processing
X	0008380	biological_process	RNA splicing
X	0030620	molecular_function	U2 snRNA binding
X	0071004	cellular_component	U2-type prespliceosome
X	1903241	biological_process	U2-type prespliceosome assembly
X	1990904	cellular_component	ribonucleoprotein complex
Y	0000398	biological_process	mRNA splicing, via spliceosome
Y	0003674	molecular_function	molecular_function
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005681	cellular_component	spliceosomal complex
Y	0005686	cellular_component	U2 snRNP
Y	0005737	cellular_component	cytoplasm
Y	0006397	biological_process	mRNA processing
Y	0008380	biological_process	RNA splicing
Y	0030620	molecular_function	U2 snRNA binding
Y	0071004	cellular_component	U2-type prespliceosome
Y	1903241	biological_process	U2-type prespliceosome assembly
Z	0000398	biological_process	mRNA splicing, via spliceosome
Z	0003674	molecular_function	molecular_function
Z	0003723	molecular_function	RNA binding
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005681	cellular_component	spliceosomal complex
Z	0005684	cellular_component	U2-type spliceosomal complex
Z	0005737	cellular_component	cytoplasm
Z	0006397	biological_process	mRNA processing
Z	0008380	biological_process	RNA splicing
Z	0071013	cellular_component	catalytic step 2 spliceosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue GTP C 1500

Chain	Residue
C	LEU142
C	ASP271
C	ARG272
C	SER315
C	THR316
C	LYS317
C	MG1501
C	HIS143
C	GLY145
C	LYS146
C	THR147
C	SER148
C	SER190
C	GLY217
C	LYS269

---

site_id	AC2
Number of Residues	4
Details	binding site for residue MG C 1501

Chain	Residue
C	THR147
C	SER190
C	ASP214
C	GTP1500

---

site_id	AC3
Number of Residues	5
Details	binding site for residue ZN Q 400

Chain	Residue
Q	CYS13
Q	CYS16
Q	CYS71
Q	CYS73
Q	CYS74

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN Q 401

Chain	Residue
Q	CYS34
Q	CYS37
Q	CYS61
Q	CYS64

---

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN R 400

Chain	Residue
R	CYS73
R	CYS81
R	CYS87
R	HIS91

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN T 1000

Chain	Residue
T	CYS104
T	CYS105
T	CYS108
T	CYS148

---

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN T 1001

Chain	Residue
T	CYS104
T	CYS122
T	CYS150
T	CYS153

---

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN T 1002

Chain	Residue
T	CYS108
T	CYS120
T	CYS122
T	CYS145

---

site_id	AC9
Number of Residues	4
Details	binding site for residue MG B 200

Chain	Residue
B	G99
E	G78
E	U80
b	U1

---

site_id	AD1
Number of Residues	3
Details	binding site for residue MG E 201

Chain	Residue
E	A59
E	A79
E	U80

---

site_id	AD2
Number of Residues	1
Details	binding site for residue MG E 202

Chain	Residue
E	G81

---

site_id	AD3
Number of Residues	1
Details	binding site for residue MG E 203

Chain	Residue
E	G81

---

site_id	AD4
Number of Residues	2
Details	binding site for residue MG E 204

Chain	Residue
E	C61
E	G77

---

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. IATAgrDsVIKLWDM

Chain	Residue	Details
O	ILE239-MET253
O	VAL281-VAL295
n	ILE178-PHE192

---

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ScVIIDEAHE

Chain	Residue	Details
e	SER468-GLU477

---

site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqknEtNngstCiCRVP

Chain	Residue	Details
T	LEU103-PRO125

---

site_id	PS00998
Number of Residues	10
Details	G10_2 G10 protein signature 2. CvHCGCRGCA

Chain	Residue	Details
T	CYS145-ALA154

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	490
Details	Region: {"description":"Reverse transcriptase homology domain"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	273
Details	Region: {"description":"Linker"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	13
Details	Region: {"description":"Important for branch point selection"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	171
Details	Region: {"description":"Restriction endonuclease homology domain"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	207
Details	Domain: {"description":"tr-type G","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	7
Details	Region: {"description":"G1","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Region: {"description":"G2","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Region: {"description":"G3","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	Region: {"description":"G4","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Region: {"description":"G5","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	14
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	27
Details	Compositional bias: {"description":"Polar residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	70
Details	Repeat: {"description":"WD 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	69
Details	Repeat: {"description":"WD 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	30
Details	Repeat: {"description":"WD 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	30
Details	Repeat: {"description":"WD 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	29
Details	Repeat: {"description":"WD 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	29
Details	Repeat: {"description":"WD 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	30
Details	Repeat: {"description":"WD 7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	93
Details	Domain: {"description":"RRM","evidences":[{"source":"PROSITE-ProRule","id":"PRU00176","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	27
Details	Zinc finger: {"description":"C3H1-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00723","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	9
Details	Motif: {"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	182
Details	Domain: {"description":"MIF4G","evidences":[{"source":"PROSITE-ProRule","id":"PRU00698","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	117
Details	Domain: {"description":"MI","evidences":[{"source":"PROSITE-ProRule","id":"PRU00698","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	47
Details	Domain: {"description":"HTH myb-type 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00625","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	45
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU00625","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	32
Details	Repeat: {"description":"HAT 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	32
Details	Repeat: {"description":"HAT 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI31
Number of Residues	32
Details	Repeat: {"description":"HAT 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI32
Number of Residues	31
Details	Repeat: {"description":"HAT 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI33
Number of Residues	31
Details	Repeat: {"description":"HAT 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI34
Number of Residues	34
Details	Repeat: {"description":"HAT 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI35
Number of Residues	58
Details	Region: {"description":"Interaction with CEF1","evidences":[{"source":"PubMed","id":"15601865","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI36
Number of Residues	144
Details	Domain: {"description":"Sm","evidences":[{"source":"PROSITE-ProRule","id":"PRU01346","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI37
Number of Residues	21
Details	Repeat: {"description":"LRR 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI38
Number of Residues	166
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI39
Number of Residues	3
Details	Motif: {"description":"DEAH box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI40
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---