5WLY

E. coli LpxH- 8 mutations

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	UDP-2,3-diacylglucosamine hydrolase	polymer	248	27732.0	1	UniProt (Q0P6L0) Pfam (PF00149) UniProt (by SIFTS) (P43341)	Escherichia coli	UDP-2,3-diacylglucosamine diphosphatase
2	B, C (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	E (A)	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
5	F (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	G (A)	water	water		18.0	149	Chemie (HOH)

Sequence modifications

A: 1 - 240 (UniProt: Q0P6L0)

PDB	External Database	Details
Ala 14	Glu 14	engineered mutation
Ala 15	Glu 15	engineered mutation
His 141	Phe 141	engineered mutation
Ser 142	Leu 142	engineered mutation
Ser 146	Leu 146	engineered mutation
His 147	Phe 147	engineered mutation
Thr 161	Lys 161	engineered mutation
Ala 162	Glu 162	engineered mutation
Leu 241	-	expression tag
Glu 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27732.0
Non-Polymers*	Number of molecules	5
	Total formula weight	253.4
All*	Total formula weight	27985.4

*Water molecules are not included.