5WJ4

Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	241	26934.4	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	C, D (A, B)	water	water		18.0	547	Chemie (HOH)

Sequence modifications

A, B: 2 - 236 (UniProt: P42212)

PDB	External Database	Details
Gly -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Cr2 65	Ser 65	chromophore
Cr2 65	Tyr 66	chromophore
Cr2 65	Gly 67	chromophore
Ala 67	Gln 69	engineered mutation
Ser 97	Phe 99	engineered mutation
Thr 103	Asn 105	engineered mutation
Phe 143	Tyr 145	engineered mutation
Cys 145	Ser 147	engineered mutation
Asp 146	His 148	engineered mutation
Thr 151	Met 153	engineered mutation
Ala 161	Val 163	engineered mutation
Val 169	Ile 171	engineered mutation
Val 201	Thr 203	engineered mutation
Cys 202	Gln 204	engineered mutation
Gln 220	Glu 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53868.8
All*	Total formula weight	53868.8

*Water molecules are not included.