5WJ4
Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 134.570, 134.570, 69.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.185 - 1.631 |
R-factor | 0.1651 |
Rwork | 0.164 |
R-free | 0.19310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qyq |
RMSD bond length | 0.016 |
RMSD bond angle | 1.365 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.190 | 39.190 | 1.660 |
High resolution limit [Å] | 1.630 | 8.930 | 1.630 |
Rmerge | 0.113 | 0.052 | 2.115 |
Rmeas | 0.117 | 0.054 | 2.195 |
Rpim | 0.031 | 0.015 | 0.584 |
Number of reflections | 79222 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 100.0 | 98.8 | 99.9 |
Redundancy | 14.6 | 12.1 | 14 |
CC(1/2) | 0.999 | 0.997 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350 |