5WJ3

Crystal structure of green fluorescent protein Clover mutant S147C/Q204C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Green fluorescent protein	polymer	241	26997.4	2	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2		water	water		18.0	741	Chemie (HOH)

Sequence modifications

A, B: 3 - 238 (UniProt: P42212)

PDB	External Database	Details
Gly -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Ser 2	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 69	Gln 69	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Cys 147	Ser 147	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
His 203	Thr 203	engineered mutation
Cys 204	Gln 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53994.9
All*	Total formula weight	53994.9

*Water molecules are not included.