5WJ3
Crystal structure of green fluorescent protein Clover mutant S147C/Q204C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.620, 82.028, 92.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.015 - 1.351 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.16220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 3.660 | 1.350 |
| Rmerge | 0.052 | 0.032 | 0.463 |
| Rmeas | 0.057 | 0.035 | 0.500 |
| Rpim | 0.021 | 0.014 | 0.187 |
| Total number of observations | 788958 | ||
| Number of reflections | 110794 | ||
| <I/σ(I)> | 14.1 | ||
| Completeness [%] | 97.1 | 93.9 | 94.7 |
| Redundancy | 7.1 | 6.4 | 6.9 |
| CC(1/2) | 0.999 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350 |
