5WJ3
Crystal structure of green fluorescent protein Clover mutant S147C/Q204C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.620, 82.028, 92.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.015 - 1.351 |
R-factor | 0.143 |
Rwork | 0.142 |
R-free | 0.16220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qyq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 3.660 | 1.350 |
Rmerge | 0.052 | 0.032 | 0.463 |
Rmeas | 0.057 | 0.035 | 0.500 |
Rpim | 0.021 | 0.014 | 0.187 |
Total number of observations | 788958 | ||
Number of reflections | 110794 | ||
<I/σ(I)> | 14.1 | ||
Completeness [%] | 97.1 | 93.9 | 94.7 |
Redundancy | 7.1 | 6.4 | 6.9 |
CC(1/2) | 0.999 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350 |