5WIE

Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, G)	Voltage-gated potassium channel subunit beta-2	polymer	333	37353.1	2	UniProt (P62483) Pfam (PF00248)	Rattus norvegicus (Rat)	K(+) channel subunit beta-2,Kv-beta-2
2	B, D (B, H)	Potassium voltage-gated channel subfamily A member 2,Potassium voltage-gated channel subfamily A member 2	polymer	532	60455.3	2	UniProt (P63142) UniProt (P63141) Pfam (PF02214) Pfam (PF00520)	Rattus norvegicus (Rat)	RAK,RBK2,RCK5,Voltage-gated potassium channel subunit Kv1.2,MK2,Voltage-gated potassium channel subunit Kv1.2
3	E, O (A, G)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
4	F, G, H, I, J... (B, H)	(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate	non-polymer		749.0	6	Chemie (PGW)
5	K, L, M, N, Q... (B, H)	POTASSIUM ION	non-polymer		39.1	8	Chemie (K)

Sequence modifications

A, G: 36 - 367 (UniProt: P62483)

PDB	External Database	Details
Met 35	-	initiating methionine

B, H: 1 - 42 (UniProt: P63142)

PDB	External Database	Details
Met -36	-	initiating methionine
Ser -35	-	expression tag
Ala -34	-	expression tag
Trp -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
Pro -30	-	expression tag
Gln -29	-	expression tag
Phe -28	-	expression tag
Glu -27	-	expression tag
Lys -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ala -14	-	expression tag
Trp -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Pro -10	-	expression tag
Gln -9	-	expression tag
Phe -8	-	expression tag
Glu -7	-	expression tag
Lys -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 15	Leu 15	linker
Ser 31	Cys 31	linker
Ser 32	Cys 32	linker
Gln 207	Asn 207	linker
Tyr 266	-	linker
Tyr 267	-	linker
Val 268	-	linker
Thr 269	-	linker
Ile 270	-	linker
Phe 271	-	linker
Leu 272	-	linker
Thr 273	-	linker
Glu 274	-	linker
Ser 275	-	linker

B, H: 43 - 56 (UniProt: P63141)

PDB	External Database	Details
Asn 276	Asp 280	conflict
Lys 277	Ala 281	conflict
Ser 278	Gln 282	conflict
Val 279	Gln 283	conflict
Leu 280	Gly 284	conflict
Phe 282	Gln 286	conflict
Gln 283	Ala 287	conflict
Asn 284	Met 288	conflict
Val 285	Ser 289	conflict
Arg 286	Leu 290	conflict
Arg 287	Ala 291	conflict
Val 288	Ile 292	conflict
Val 289	Leu 293	conflict
Gln 290	Arg 294	conflict
Ile 291	Val 295	conflict
Phe 292	Ile 296	conflict
Ile 294	Leu 298	conflict
Met 295	Val 299	conflict
Ile 297	Val 301	conflict
Leu 298	Phe 302	conflict
Trp 406	Val 410	engineered mutation
Ser 431	Cys 435	conflict
Ser 478	Cys 482	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	195616.8
Non-Polymers*	Number of molecules	16
	Total formula weight	6293.6
All*	Total formula weight	201910.4

*Water molecules are not included.