5WG1

Kelch domain of human Keap1 bound to mutant Nrf2 EAGE peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Kelch-like ECH-associated protein 1	polymer	336	36899.2	2	UniProt (Q14145) Pfam (PF01344) In PDB	Homo sapiens (Human)	Cytosolic inhibitor of Nrf2,INrf2,Kelch-like protein 19
2	P	Nrf2 EAGE mutant peptide	polymer	9	1022.1	1		Homo sapiens
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	56	Chemie (HOH)

Sequence modifications

A, B: 320 - 612 (UniProt: Q14145)

PDB	External Database	Details
Met 289	-	initiating methionine
Gly 290	-	expression tag
Ser 291	-	expression tag
Ser 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
Ser 299	-	expression tag
Ser 300	-	expression tag
Gly 301	-	expression tag
Gly 302	-	expression tag
Glu 303	-	expression tag
Asn 304	-	expression tag
Leu 305	-	expression tag
Tyr 306	-	expression tag
Phe 307	-	expression tag
Gln 308	-	expression tag
Gly 309	-	expression tag
His 310	-	expression tag
Met 311	-	expression tag
Lys 312	-	expression tag
Pro 313	-	expression tag
Thr 314	-	expression tag
Gln 315	-	expression tag
Val 316	-	expression tag
Met 317	-	expression tag
Pro 318	-	expression tag
Ser 319	-	expression tag
Ala 540	Glu 540	engineered mutation
Ala 542	Glu 542	engineered mutation
Ser 613	-	expression tag
Arg 614	-	expression tag
Lys 615	-	expression tag
Gln 616	-	expression tag
Ile 617	-	expression tag
Asp 618	-	expression tag
Gln 619	-	expression tag
Gln 620	-	expression tag
Asn 621	-	expression tag
Ser 622	-	expression tag
Thr 623	-	expression tag
Ser 624	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	74820.4
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	75012.5

*Water molecules are not included.