5WFN

Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Actin-5C	polymer	384	42886.8	2	UniProt (P10987) Pfam (PF00022)	Drosophila melanogaster (Fruit fly)
2	B, D (C, D)	Leiomodin-2	polymer	506	56753.7	2	UniProt (Q6P5Q4) Pfam (PF03250)	Homo sapiens (Human)	Cardiac leiomodin, C-LMOD, Leiomodin
3	E, G (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
4	F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 0 - 375 (UniProt: P10987)

PDB	External Database	Details
Glu 291	Lys 292	engineered mutation
Lys 322	Pro 323	engineered mutation
Ala 376	-	expression tag
Ser 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag

C, D: 33 - 128 (UniProt: Q6P5Q4)

PDB	External Database	Details
Met 22	-	expression tag
Ala 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
Val 30	-	expression tag
Gly 31	-	expression tag
Thr 32	-	expression tag

C, D: 129 - 440 (UniProt: Q6P5Q4)
C, D: 441 - 547 (UniProt: Q6P5Q4)

PDB	External Database	Details
Gly 478	Lys 478	engineered mutation
Ser 479	Lys 479	engineered mutation
Gly 480	Lys 480	engineered mutation
Ser 481	Lys 481	engineered mutation
Gly 483	Lys 483	engineered mutation
Ser 484	Lys 484	engineered mutation
Gly 486	Lys 486	engineered mutation
Ser 487	Lys 487	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	199281.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	200342.0

*Water molecules are not included.