5WFN
Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-07-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 65.350, 65.650, 81.920 |
Unit cell angles | 101.29, 90.94, 107.97 |
Refinement procedure
Resolution | 44.245 - 3.000 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.24630 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.888 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.070 | |
High resolution limit [Å] | 2.980 | 2.980 |
Number of reflections | 25186 | |
Completeness [%] | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PEG3350, 0.2 M magnesium acetate |